M. Fattahi; V. Nazeri; F. Sefidkon; Z. Zamani
Abstract
Study on relationships between a species with its surrounding biotic and abiotic environment provides valuable information in terms of propagation, domestication and breeding. For this purpose, the autecology studies of Dradocephalum kotschyi Bioss was studied in 12 natural habitats. Natural habitats ...
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Study on relationships between a species with its surrounding biotic and abiotic environment provides valuable information in terms of propagation, domestication and breeding. For this purpose, the autecology studies of Dradocephalum kotschyi Bioss was studied in 12 natural habitats. Natural habitats were determined with using Flora Iranica and with the assistance of local people. Also, topography and climate conditions were determined for each natural habitat. In addition, phenology and dominant companion species were recorded in all growing regions. Other studied traits were mostly growth and reproductive ones including height, basal diameter and canopy cover which were evaluated in situ. Plant fitness was evaluated based on the number of produced seeds, flowering stems, observed expected and produced seeds. Results of topography and climate data showed that natural habitats were mostly in rocky highlands with a high relative humidity and cool weather in summer. Plant fitness and growth condition were significantly different in different regions which could be due to ecological and/ or genetic variations. According to the results, domestication and breeding programs are urgently required for the conservation of this valuable but endangered species.
A.R. Yavari; V. Nazeri; F. Sefidkon; Z. Zamani; M.E. Hassani
Abstract
In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. ...
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In the present study, the genetic variation within and among some populations of Thymus migricus Klokov & Desj.-Shost was investigated using the RAPD markers. Twenty-one decamer RAPD primers produced 310 unique bands. RAPD analysis showed 14 monomorphic and 296 polymorphic bands in different genotypes. The number of polymorphic bands per primer varied from 5 to 20 with a mean of 14.09. Genetic distance was measured by Nei’s coefficient and cluster analysis was carried out. A dendrogram was drawn based on genetic distance data, applying the UPGMA clustering method. Analysis of molecular variance (AMOVA) was performed. According to the dendrogram, among T. migricus populations, Band and Jolfa populations had maximum differences with a distance of 0.130. Evaluation of genetic diversity within populations with an average of Nei’s gene diversity analysis and Shannon’s information index, showed that diversity within population of Jolfa (h = 0.196 & I = 0.294) was more than other populations while genetic diversity within population of Ghushchi (h = 0.139 & I = 0.209) was less than other populations. Mean of Fst and Nm indexes which show gene flow among populations, were 0.30 and 1.14, indicating a greater gene flow among five populations of T. migricus. The results of the present study showed that there was a greater level of genetic variation in the Iranian natural populations of T. migricus which could be applied for future breeding programs.
